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Image Search Results
Journal: Arthritis Research & Therapy
Article Title: Anti-PM/Scl antibodies are found in Japanese patients with various systemic autoimmune conditions besides myositis and scleroderma
doi: 10.1186/s13075-015-0573-x
Figure Lengend Snippet: Detection of anti-PM/Scl antibodies in immuoprecipitation analysis. TnT-IPP: immunoprecipitation of biotinylated recombinant PM/Scl-100 and PM/Scl-75. Recombinant proteins were subjected to 4% to 20% SDS-PAGE and analyzed by immunoblotting with streptavidin-alkaline phosphatase and substrate. In., the input was half the dose for immunoprecipitation. Lanes A to K correspond to the anti-PM-Scl-100 and/or −75-positive patients shown in Figure . Lane N: healthy control serum. HeLa-IPP: immunoprecipitation analysis using radiolabeled HeLa cell extracts. Lanes A to L correspond to the patients shown in Figure and Table . Lanes A to K correspond to anti-PM-Scl-100 and/or −75-positive patients shown in Figure . Lane M: [Methyl- 14 C] methylated protein MW markers (PerkinElmer Japan, Yokohama, Japan). Lane L: anti-U1-RNP-positive serum with equivocal titers for both antibodies in ELISA. Lanes 1 to 4 show the reference sera; lane 1, anti-PM/Scl-positive serum; lane 2, anti-MDA5-positive serum; lane 3, anti-TIF1-γ-positive serum; lane 4, anti-Mi-2-positive serum. Arrow and arrowhead corrspond to the PM/Scl-100 and PM/Scl-75 antigens, respectively. IPP, immunoprecipitation; TnT, in vitro translation and transcription product.
Article Snippet: Lane M: [Methyl- 14 C]
Techniques: Immunoprecipitation, Recombinant, SDS Page, Western Blot, Control, Methylation, Enzyme-linked Immunosorbent Assay, In Vitro
Journal: Nutrients
Article Title: Integrated Proteomics and Metabolomics Reveal the Direct Hepatic Protection of Propionate Against Alcoholic Liver Disease via the RGN-PPARα Pathway
doi: 10.3390/nu18050872
Figure Lengend Snippet: Effect of propionate on RGN-PPARα signaling. ( A ) PPI network of DEPs between propionate-treated group and EtOH group. ( B , C ) Predicted binding modes of propionate to the active domain of RGN: ( B ) three-dimensional and ( C ) two-dimensional interaction diagrams. ( D ) Representative Western blot images of RGN, PPARα, p-AMPK, and ACOX1 protein expression in the liver. ( E ) Quantification normalized to the indicated loading controls (RGN/PPARα to β-actin; p-AMPK to AMPK; and ACOX1 to GAPDH). The data are presented as means ± SEM (n = 4). * p < 0.05; ** p < 0.01.
Article Snippet: Primary antibodies, including β-actin (#66009-1-Ig), CPT1A (#15184-1-AP), SREBP-1 (#14088-1-AP), ACC1 (#21923-1-AP), ACOX1 (#83731-2-RR), PPARα (#66826-1-Ig) and
Techniques: Binding Assay, Western Blot, Expressing